正交试验优选防感颗粒的提取工艺

Optimum Extracting Procedure of Fanggan Granules by Orthogonal Design

摘要: 目的确立防感颗粒的最佳提取工艺。方法应用正交试验和HPLC，以防感颗粒中R,S-告依春、白花前胡甲素、苦杏仁苷及甘草苷4种活性成分含量为指标，采用多指标综合评分法，确定防感颗粒的提取工艺。结果防感颗粒最佳提取工艺为：药材加8倍量水，煎煮3次，每次1.5 h，乙醇浓度为60%。结论优选出的工艺稳定可靠，可用于防感颗粒的提取。

Abstract: OBJECTIVE To establish the optimum extracting procedure of Fanggan granules. METHODS A multi index comprehensive evaluation method by orthogonal test and HPLC was used for the determination of the extracting procedure of Fanggan granules, with four active ingredients including (R,S)-epigoitrin, praeruptorin A, amygdalin and liquiritin as the content index. RESULTS The optimum extraction process of Fanggan granules was determined as follows: herbs were extracted 3 times with 8 times of water; 1.5 h each time; ethanol concentration was 60%. CONCLUSION The optimum procedure is stable and reliable for the extraction of Fanggan granules.