Abstract: OBJECTIVE To analysis berberine hydrochloride in Berberis heteropoda cortex by frared diffuse reflection optical spectra combined with PCR. METHODS Between the measured value and chemical spectroscopy, a method of NIR diffuse reflectance optic-fiber spectroscopy combined with PCR was established to analysis berberine hydrochloride in Berberis heteropoda cortex. RESULTS The root-mean-square error of cross-validation(RMSECV) of the berberine hydrochloride in Berberis heteropoda cortex was 1.016 9, the correlation coefficients was 0.979 5, the average recycle rate was 108.5%. Statistical test was used to analysis the dates and it showed that the difference between NIR prediction values and the values determinated by standard methods had no statistical significance. CONCLUSION It indicates that NIR diffuse reflectance optic-fiber spectroscopy combined with PCR method can be used in rapid determination of the contents of Berberis heteropoda Schrenk.