Abstract: OBJECTIVE To develop an HPLC method for simultaneous determination of four flavonoids(myricetin, quercetin, kaempferol, isorhamnetin) in different parts of Cedrus deodara. METHODS The sample were pretreated, including reflux extraction with 60% methanol for 1 h, and then extracting solution hydrolysis with 10% hydrochloric acid for another 1 h. Agilent TC-C₁₈(250 mm×4.6 mm, 5 μm) column was adopted. The mobile phase was acetonitrile-0.1% phosphoric acid solution with the gradient elution at the flow rate of 1.0 mL·min-1. The column temperature was 30 ℃ and the detection wavelength was 360 nm. RESULTS Good linear correlation were found in the ranges of 0.5-10.0 μg·mL-1 for myricetin, quercetin and isorhamnetin and 1-24.0 μg·mL-1 for kaempferol(r≥0.999 6). The average recoveries(n=6) of the four flavonoids were 98.9%, 100.1%, 99.5% and 101.7%, respectively. The results showed that the concents of four flavonoids in different parts of C. deodara were significant different, the highest contents of quercetin and kaempferol of pine needles were 0.285 mg·g-1 and 1.493 mg·g-1, the highest contents of myricetin and isorhamnetin of tender branchlets were 0.365 mg·g-1 and 0.476 mg·g-1 respectively. The order of total contents of four flavonoids from highest to lowest were pine needles, tender branchlets, inflorescences, woods, barks. CONCLUSION The method is simple, rapid and efficient, and applicable for determination of four flavonoids in different parts of C. deodara.