Abstract: OBJECTIVE To study the cardioprotective effect of ginkgolide B(GB) against myocardial ischemia-reperfusion injury(MIRI) in rats. METHODS All of 100 rats were randomly allocated into five groups: sham group(n=20), model group(n=20), GB low, medium and high dosage groups(for each group, n=20). Previous to the operation, rats were orally administrated with drugs for continuous 7 days. During the operation, the left anterior descending coronary artery was ligated for 30 min before reperfusion, except for the rats in sham groups. After 48 h, myocardial function was detected, followed with measuring the infracted areas. The degree of oxidative stress around the damaged area was analyzed by immunohistochemical staining. The expression of apoptosis-related proteins Bcl-2 and Bax, combined with the phosphorylation level of pro-inflammation proteins Erk, Jnk and p38 were detected with PCR or Western blot. RESULTS GB preconditioning significantly alleviated MIRI and post-injury myocardial function. Compared with model group, the phosphorylation level of pro-inflammation proteins were significantly decreased, as well as the oxidative stress level around coronary tissue measured with DHE staining. CONCLUSION In MIR model of rats, GB pre-treatment can significantly protect myocardial damage by anti-oxidative and anti-apoptosis comprehensive pathways.