Abstract: OBJECTIVE To investigate the different parameters influenced on the process of crystallization of dioscorea and determine the optimal conditions. METHODS Based on the crystal purity and precipitation rate, the influences of crystallization temperature, crystallization time, cooling rate and stirring rate on product were investigated by single factor experiment. RESULTS When the crystallization temperature was 30 ℃, the holding time was 2 h, cooling rate was 33-50 ℃?h^-1 and the stirring speed was 60 r?min^-1, the product purity could achieve more than 98% and the precipitation rate was 75%-80%. CONCLUSION The method is reliable and stable, and can provide reference data for industrial production.