杨梅酮对人肺腺癌A549细胞凋亡和细胞周期的影响

Effect of Myricanone on Cell Apoptosis and Cell Cycle in Human Lung Adenocarcinoma A549 Cells

摘要: 目的检测杨梅酮对体外人肺腺癌细胞A549细胞凋亡和细胞周期的影响。方法采用流式细胞仪分别检测各组的细胞早期凋亡率和细胞周期百分率。结果杨梅酮能显著增加A549细胞早期凋亡率和G₀/G₁期的细胞比例，并且细胞凋亡率与杨梅酮浓度具有较好的量效关系。结论杨梅酮能显著增加体外人肺腺癌细胞A549细胞早期凋亡率，并可使A549细胞周期阻滞在G₀/G₁期。

Abstract: OBJECTIVE To detect the effect of myricanone on cell apoptosis and cell cycle in human lung adenocarcinoma A549 cells. METHODS The early cell apoptosis rate and cell cycle percentage of each group were detected by flow cytomytry. RESULTS Myricanone induced early cell apoptosis of A549 cells in a dose-dependent manner, as well as increased the percentage of A549 cells blocked in G₀/G₁ stage. CONCLUSION Myricanone could significantly increase the cell apoptosis rate of A549 cells and could block cell cycle of A549 cells in G₀/G₁ stage.