Abstract: OBJECTIVE To establish a UV method to determine the content of total alkaloids of Fritiliariae Thunbergh Bulbus and compare its difference from different habitats. METHODS Acid dye colorimetry was applied to determine by using UV spectrophotometry with peimine as control. RESULTS Total alkaloids had a good linear relationship in 0.059 2-0.295 9 mg·mL^-1. Its accuracy (97.74%), reproducibility(RSD=2.05%), precision(RSD=3.22%) showed a good repeatability. The total alkaloids content of Fritiliariae Thunbergh Bulbus from different habitats were Panan 0.53%, Jinyun 0.52%, Xiangshan 0.52%, Dongyang 0.44%, Yinzhou 0.41%, Zhoushan 0.38%. CONCLUSION The content of total alkaloids of Fritiliariae Thunbergh Bulbus from different habitats is different, and the content is much higher in Fritiliariae Thunbergh Bulbus from Panan, Jinyun and Xiangshan than other habitats.