Abstract: OBJECTIVE To establish a rapid assay for detection of huperzine A based on gold immunochromatography assay. METHODS Colloidal gold marked coupled with monoclonal antibody against huperzine A was jet sprayed onto the glass fiber and stored at 4 ℃. Huperzine A and anti-mouse goat immunoglobulins were jet-positioned onto a nitrocellulose membrane. Nitrocellulose membrane, gold combined pad, sample pad and absorbent paper were assembled and cut into detecting card, respectively. RESULTS The results showed that the sensitive of rapid for huperzine A visual detection limit was 15 μg·mL^-1 and detection time within 5 min. Repeatability and specificity of strip was good. CONCLUSION It is very simple and convenient to rapidly detect huperzine A on the spot.