Abstract: OBJECTIVE To compare two methods for the determination of tulobuterol. METHODS Non-aqueous titration method was performed with acetic acid and acetic anhydride(7∶3) as the solvent and crystal violet as indicator. The tulobuterol solution was titrated with 0.1 mol·L^-1 perchloric acid solution. The end point was decided by potentiometric method and indicator method. The HPLC method was performed on a C₁₈ column(250 mm×4.6 mm, 5 μm) with mobile phase of acetonitrile-5 mmol·L^-1 phosphate buffer at flow-rate of 1 mL·min^-1. The detection was conducted at 215 nm. RESULTS There was no significant difference in accuracy between the results of HPLC and the nonaqueous titration method. CONCLUSION The results indicated that the two methods were simple, accurate and reproducible, and they both can be used to determine the content of tulobuterol.