Abstract:
OBJECTIVE To develop an HPLC method for simultaneous determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara. METHODS Agilent TC-C
18 column (250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was methanol (A)-0.2% phosphoric acid (B) with gradient elution (0-8 min, 10%A; 8-45 min, 10%→30%A) at the flow rate of 1.0 mL·min
-1. The column temperature was 25 ℃ and the detection wavelength was 280 nm. RESULTS Calibration curves were found to be linear in the ranges of 0.4-4.0 μg·mL
-1 for gallic acid, 4-40 μg·mL
-1 for protocatechuic acid and 2-20 μg·mL
-1 for catechin. The correlation coefficients were 0.999 8, 0.999 9, 0.999 9. The average recoveries(n=9) of gallic acid, protocatechuic acid and catechin were 104.7%, 99.5% and 105.8%. CONCLUSION The method is simple, rapid and efficient, which is suitable for the determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara.