HPLC同时测定雪松松针中没食子酸、原儿茶酸和儿茶素的含量

    Simultaneous Determination of Gallic Acid, Protocatechuic Acid and Catechin in Pine Needles of Cedrus Deodara by HPLC

    • 摘要: 目的 建立同时测定雪松松针中没食子酸、原儿茶酸和儿茶素含量的高效液相色谱法。方法 采用TC-C18(250 mm× 4.6 mm,5 μm)色谱柱,流动相为甲醇(A)-0.2%磷酸水溶液(B)梯度洗脱(0~8 min,10%A,8~45 min,10%→30%A),流速:1.0 mL·min-1,检测波长:280 nm,柱温:25 ℃。结果 雪松松针中没食子酸、原儿茶酸和儿茶素依次在0.4~4.0,4~40和2~20 μg·mL-1内呈良好线性关系,相关系数r分别为0.999 8,0.999 9,0.999 9,加样回收率(n=9)分别为104.7%,99.5%和105.8%。结论 该方法适用于雪松松针中没食子酸、原儿茶酸和儿茶素的含量测定,方法简单、快速、高效。

       

      Abstract: OBJECTIVE To develop an HPLC method for simultaneous determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara. METHODS Agilent TC-C18 column (250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was methanol (A)-0.2% phosphoric acid (B) with gradient elution (0-8 min, 10%A; 8-45 min, 10%→30%A) at the flow rate of 1.0 mL·min-1. The column temperature was 25 ℃ and the detection wavelength was 280 nm. RESULTS Calibration curves were found to be linear in the ranges of 0.4-4.0 μg·mL-1 for gallic acid, 4-40 μg·mL-1 for protocatechuic acid and 2-20 μg·mL-1 for catechin. The correlation coefficients were 0.999 8, 0.999 9, 0.999 9. The average recoveries(n=9) of gallic acid, protocatechuic acid and catechin were 104.7%, 99.5% and 105.8%. CONCLUSION The method is simple, rapid and efficient, which is suitable for the determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara.

       

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