Abstract: OBJECTIVE To investigate the wound healing of biological medical glue ameliorating wound tissue regeneration and wound healing. METHODS The healthy SD rat were allowed into 3 group as follows: polysaccharide medical biological glue group, saline group, povidone iodine solution group. The model were record after injury 3, 7, 14 and 28 d wound area, and the wound repair tissue were carried out pathological check at 7, 14 and 28 d. RESULTS After injury 7, 14, 28 d, the wound area in polysaccharide biological medical glue group were significantly decreased(P<0.05). HE staining showed that polysaccharide biological medical glue could promote the wound healing pathological process, fibroblasts in the polysaccharide biological medical glue group generally increased significantly. CONCLUSION Polysaccharide biological medical glue can promote the wound healing in rat.