β-环糊精手性流动相添加剂法测定爱维莫潘中的异构体

Separation of Alvimopan and Its Enantiomers by HPLC Using β-cyclodextrin as Chiral Mobile Phase Additive

摘要: 目的建立爱维莫潘的高效液相手性拆分分析法。方法选用C₁₈柱(4.6 mm×250 mm，5 μm)，流动相为水溶液(含0.5%冰醋酸，0.5%三乙胺，1%β-环糊精)-甲醇(80∶20)，柱温为40 ℃，检测波长为275 nm。结果爱维莫潘和它的3个异构体都达到良好分离，分离度符合要求。结论本方法具有专属性强，灵敏度高，重复性良好，耐用性好，简便快捷，使用成本低廉等优点。

Abstract: OBJECTIVE To separate the enantiomers of alvimopan by HPLC which was developed using chiral mobile phase. METHODS Separation was performed on a C₁₈ column(4.6 mm×250 mm, 5 μm). The mobile phase was methanol- water(concentration of 0.5% glacial acetic acid, 0.5% triethylamine and 1%β-cyclodextrin)(20∶80). The detection wavelength was set at 275 nm and the temperature was 40 ℃. RESULTS With this method, alvimopan and its enantiomers were well separated, the resolution met the requirement. CONCLUSION The method is simple, specific, sensitive, repeatable accurate and inexpensive.