Abstract: OBJECTIVE To investigate the effect of sevoflurane on the expression of non-receptor tyrosine kinases steroid receptor coactivator(Src) and focal adhesion kinase(FAK) in diabetic rats after renal ischemia/reperfusion(I/R) injury. METHODS The well established streptozotocin (STZ)-induced diabetes mellitus(DM) model was used. Male rats were randomly assigned to three groups: sham group, I/R group and sevoflurane pretreatment group. After 24 h, serum creatinine(Cr), blood urea nitrogen(BUN) levels, renal Src and FAK expression(immunohistochemistry) were assessed. RESULTS Compared with sham group, the Cr and BUN levels of rats in I/R group were significantly higher, while the Cr and BUN levels of rats in sevoflurane pretreatment group were significantly lower than those of rats in I/R group. The Src and FAK expression of rats in renal I/R group were significantly lower than those of rats in sham group, while the Src and FAK expression in sevoflurane pretreatment group were significantly higher than those of rats in renal I/R group. CONCLUSION Sevoflurane pretreatment in rats with diabetes mellitus protects the kidneys from I/R injury and increases renal Src and FAK expression.