Abstract: OBJECTIVE To establish a quantitative determination method for hederagenin in Akebia Fructus after being hydrolyzed by HPLC-ELSD, and to compare the content of hederagen in Akebiae Fructus different parts. METHODS Samples were analyzed with Ultimate XB column(4.6 mm×250 mm, 5 μm). Acetonitrile-water-formic acid(70∶30∶0.1) was used as mobile phases. Flow rate was 1.0 mL·min^-1. Column temperature was 35 ℃. Drift tube temperature of ELSD was set at 90 ℃. And flow rate of nitrogen was maintained 2.5 L·min^-1. RESULTS There were good linear relationship between natural logarithm of peak areas and injection quality at the range of 0.36-3.60 μg(r=0.999 4 ). The average recovery of hederagenin was 99.04% and RSD was 2.0%. The content of hederagenin in fruit, seed and peel was 24.28-35.18, 37.21-58.38, 7.83-19.44 mg·g^-1, respectively. CONCLUSION The method is simple, accurate and specific. It provides a reliable way for evaluating the quality of Akebiae Fructus. The contents of hederagenin in different medicinal parts of Akebiae Fructus were significantly different. The study provides the scientific evidence for using Akebiae Fructus.