Abstract: OBJECTIVE To establish the HPLC characteristic chromatogram of Formula Granule of Artemisiae Scopariae Herba. METHODS The chromatograms were obtained using Megres5-C₁₈ (250 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile and 0.1% phosphoric acid solution in gradient elution at flow rate of 1.0 mL·min^-1, detection wavelength of 327 nm, column temperature of 30 ℃ and sample volume of 10 μL. RESULTS The HPLC characteristic chromatogram of Formula of Artemisiae Scopariae Herba consisted of 8 common peaks. The similarity of 11 batches of samples was within 0.963-0.998. CONCLUSION The HPLC method is proved to be accurate, stable and reproducible, and it can provide reference for the identification and quality control of Formula Granule of Artemisiae Scopariae Herba.