HPLC测定银杏叶制剂中6-羟基犬尿喹啉酸的含量

Determination of 6-Hydroxykynurenic Acid in Ginkgo Preparation by HPLC

摘要: 目的建立银杏叶制剂中6-羟基犬尿喹啉酸(6-HKA)含量的测定方法。方法色谱柱为XDB-C₈(250 mm×4.6 mm，5 μm)；流动相为0.1%磷酸-乙腈-甲醇(94∶5∶1)；流速1.0 mL·min^-1；检测波长为350 nm。制剂样品经粉碎后，用50%甲醇溶解，过滤后进样测定。结果 6-HKA在5.025~100.5 μg·mL^-1内线性良好，峰面积与进样量呈良好线性关系，r=1.000 0；平均回收率为99.86%。6批银杏叶片剂中6-HKA的含量为0.22%~0.35%。结论建立的方法操作简便，结果准确，重复性好。

Abstract: OBJECTIVE To establish the method of determining the 6-hydroxykynurenic acid (6-HKA) content with HPLC. METHODS XDB-C₈ column(4.6 mm×250 mm, 5 μm) was used. Mobile phase was mixture of 0.1% phosphoric acid-CH₃CN-CH₃OH(94∶5∶1). The flow rate was 1.0 mL·min^-1. The UV detector was operated at 350 nm. Tablets were comminution, dissolution with 50% methanol, and determination after filtration. RESULTS The linear range of 6-HKA was 5.025-100.5μg·mL^-1(r=1.000 0); the average recovery was 99.86%. The content of 6-HKA in 6 batches of Ginkgo tablets was 0.22%-0.35%. CONCLUSION The method is simple, the result is accurate, and the reproducibility is good.