Abstract: OBJECTIVE To investigate the effect of the total saponins of Panax notoginseng leaves(PnGL) on blood lipid levels, liver function and lipid peroxidation in experimental hyperlipidemia rats by fat emulsion. METHODS Spraggs Dawley rats were administered with fat emulsion to establish hyperlipidemia model. The treatment groups were simultaneously administered with PnGL for 4 weeks. Serum total cholesterol(TC), triglycerides(TG), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol(LDL-c), alanine aminotransferase(ALT), aspartate aminotransferase(AST) were detected. After giving PnGL for 10 weeks, serum and liver tissue super oxide diastase(SOD), paraformaldehyde(MDA) were determined. RESULTS Both 100 mg and 200 mg PnGL could reduce the level of the serum TC, LDL-c, ALT, AST and increase HDL-c/TC ratio in rats. Simultaneously, the two doses could increase the vitality of SOD in the serum and liver tissue, and decrease the MDA level. CONCLUSION The blood lipid levels and liver function in hyperlipidemia rat could be regulated by PnGL, and also have certain resistance of lipid peroxidation. It is indicated that PnGL plays a positive role in the prevention and treatment of hyperlipidemia.