HPLC测定人血清中多索茶碱的浓度

Determination of Doxofylline in Human Serum by HPLC

摘要: 目的建立高效液相色谱测定多索茶碱血药浓度的方法。方法用乙醚提取血样，以卡马西平为内标进行检测。色谱柱采用Waters C₁₈(3.9 mm×150 mm，5 μm)，流动相为甲醇-水(35∶65)，流速为0.6 mL·min^?1，检测波长为273 nm，柱温为30 ℃。结果多索茶碱血清浓度在0.7~28 μg·mL^?1内线性关系良好，线性方程为Y=0.354 9X+0.003 9(r=0.999 7)，方法回收率为100.4%，日内、日间RSD分别为1.10%~3.87% 和2.11%~4.86%(n=5)。结论该方法快速、简便、准确、安全，可用于临床多索茶碱血药浓度的测定。

Abstract: OBJECTIVE To establish an HPLC method for determination of doxofylline in human serum. METHODS Ethyl ether was used as the exacted solvent, with carbamazepine as the internal standard. The column was Waters-C₁₈ (3.9 mm×150 mm, 5 μm). The mobile phase was methanol-water(35∶65) and the flow rate was 0.6 mL·min^?1. The detection wavelength was 273 nm. The column temperature was kept at 30 ℃. RESULTS It showed a good linear range of 0.7-28 μg·mL^?1 for doxofylline, the linear regression equation was Y=0.354 9X+0.003 9(r=0.999 7). The methodological recovery was 100.4%, the intra-day RSD and the inter-day RSD were 1.10%-3.87% and 2.11%-4.86%(n=5). CONCLUSION The method is proved to be rapid, simple, accurate and safe. It is suitable for determination of concentration of doxofylline in clinic.