Abstract: OBJECTIVE To study the protective effects and mechanism of Tengcha flavonoids(TCF) on focal cerebral ischemia reperfusion injury in rats. METHODS Ninety-six Wistar rats were randomly divided into sham group, model group, nimodipine group(10 mg·kg^-1·d^-1, TCF low dose, middle dose, high dose group(20, 40 and 80 mg·kg^-1·d^-1). Control group and model group were given the same volume of physiological saline, once a day, for gavage 5 days. Cerebral ischemia model was established in rats through occluding the middle cerebral artery (MCAO). After ischemia for 2 h, all animals were reperfused for 24 h and were killed. Behavior and infarct size were evaluated, and malondi-aldehyde(MDA) content and superoxide dismutase(SOD) activity of brain were observed. RESULTS Compared with the model group, the infarction area in the TCF 40 and 80 mg·kg^-1·d^-1 groups were significantly lower(P<0.05), MDA content of brain tissue of all TCF groups decreased(P<0.05) and SOD activity increased significantly(P<0.05). CONCLUSION TCF have protective effects on focal brain ischemia reperfusion injury in rats by improving state of oxidative stress and increasing activity of antioxidant enzymes.