Abstract: OBJECTIVE To prepare a spray solution for skin-protective and disinfectant spray. METHODS To develope the prescription composition and preparation process, and to conduct a quality study of character, identification, inspection, and content determination, et al. An acceleration method was used to investigate its stability, vector spray quantitative germicidal test to observe its bactericidal effect, acute oral toxicity test, stimulation test, the micronucleus test, subacute toxiciteit and corrosiveness test to evaluate the safety. RESULTS The quality test results of the sample were in line with provisions. The killing logarithm value of samples after 0.5 min reaction to Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans were >3.00. Its validity was determined to be 2 years by accelerated stability test. It was non-toxic of acute toxicity test orally, and it had no irritation to several intact skin of rabbits, a broken skin and eyes. The results of micronucleus test were negative. The subacute toxicity test of the sample showed that it was not harmful to male and female SD rats until the maximum dose was 1 000 mg·kg-1 by oral. The sample had no corrosion to stainless steel and carbon steel, while slight corrosion on aluminum and copper. CONCLUSION The formulation is reasonable in formulation, feasible in preparation technique, accurate in quality control, good in stablility and effective for multiplication of bacteria and fungus, and it is non-toxic and non-irritating.