双波长HPLC测定青阳参中青阳参苷元和告达庭的含量

Determination of Cynanchogenin and Caudatin in Cynanchum Otophyllum Scheid by HPLC under Double UV Wavelengths

摘要: 目的建立采用双波长HPLC同时测定青阳参中青阳参苷元和告达庭含量的方法。方法采用高效液相色谱法，ZORBAX SB-C18(4.6 mm×150 mm，5 μm)色谱柱，柱温40 ℃，流动相为乙腈-水，梯度洗脱，流速为1.0 mL·min^-1，检测波长为220，260 nm。结果青阳参苷元和告达庭均在2~64 μg·mL^-1内呈良好的线性关系，r均为1；平均回收率为100.1%和97.9%，RSD值分别为1.34%和1.37%(n=6)。结论该方法快捷简单，精密可靠，重复性好，可为青阳参及其制剂的质量控制提供参考。

Abstract: OBJECTIVE To estabish an HPLC method under double UV wavelengths for determination of cynanchogenin and caudatin in Cynanchum otophyllum Scheid. METHODS ZORBAX SB-C18 column (4.6 mm×150 mm, 5 μm) was used and the temperature of column was 40 ℃. The mobile phase consisted of methanol-acetonitrile with gradient elution. The flow rate was 1.0 mL·min^-1. The detection wavelength was set at 220 and 260 nm. RESULTS The cynanchogenin and caudatin had a good linearity relationship in 2 μg·mL^-1, with r=1. The average recoveries were 100.1% and 97.9% with RSD of 1.34% and 1.37%(n=6). CONCLUSION The method is simple, rapid, accurate and provides the reference for the quality identification of Cynanchum otophyllum Scheid and its preparation.