Abstract: OBJECTIVE To observe dynamically the effect of Shengji Yuhong ointment on deep partial-thickness burn wound healing in rats from the histopathological aspect. METHODS The model of deep partial-thickness burn rats were randomly divided into model group, Shengji Yuhong ointment group and MEBO group, 24 rats for each group. Each group were treated with corresponding drug once daily until wound healing. The time of wound healing and the wound healing rate were observed; skin collagen in the wound tissue were observed by HE staining and Masson staining on the 7th, 14th and 21st day after burn. Under the light microscope, the morphological changes of wound tissue, fibroblast morphology and quantitative changes, and the wound fibroblasts were detected. RESULTS Compared with the model group, the wound healing time shortened and wound healing rate increased in Shengji Yuhong ointment group(P<0.05). The wound fibroblast numbers in Shengji Yuhong ointment group were more than model group on the 7th, 14th day after burn(P<0.05). The wound center collagen fiber area was larger than the model group on the 14th, 21st day after burn(P<0.05). CONCLUSION Shengji Yuhong ointment has promoting effect on wound healing of deep partial-thickness burn in rat, and this may be related to the promotion of wound fibroblasts proliferation and secretion of collagen fibers.