Abstract: OBJECTIVE To investigate the reducing hemolysis effect induced by PEGylation of puerarin via in vivo. METHODS Rabbits were once-daily given the same dose of puerarin injection and PEGylated puerarin intravenously for 7 days, 3 mL blood was taken every day and used for complete blood counts analysis. At the end of 7 days of experiment, blood was taken for the determination of osmotic fragility of RBC and erythrocyte membrane fluidity. RESULTS Blood counts analysis indicated that there was no significant difference between PEGylated puerarin group and blank control group. Osmotic fragility of RBC and erythrocyte membrane fluidity indicated that PEGylated puerarin could reduce osmotic fragility of RBC and enhance erythrocyte membrane fluidity. CONCLUSION PEGylation technology could reduce intravascular hemolysis induced by puerarin, which is more safe.