Abstract: OBJECTIVE To evaluate the quality of different varieties of Chrysanthemi Flos. METHODS The content of flavonoids for different varieties of Chrysanthemi Flos which were introduced from Zhejiang Tongxiang Chrysanthemum Germplasm Nursery were determined by UV. Water vapor distillation was used to determine volatile oil content. The contents of chlorogenic acid, mignonette glycosides, and 3, 5-O-2 coffee acyl quinic acid were determined by HPLC. RESULTS The effective component in different varieties of Chrysanthemi Flos had a big difference. Compared with the cultivation of Chrysanthemi Flos, Zhejiang real estate Chrysanthemi Flos had more obvious advantages. CONCLUSION Local variety is more suitable for cultivation in the local natural conditions, it is also guaranteed authentic medicinal Chrysanthemi Flos the original advantages of the premise.