Abstract: Fatty acids are aliphatic monocarboxylic acids which act as the building blocks of lipids. They can be classified into saturated, monounsaturated and polyunsaturated fatty acids based on the number of double bonds. Fatty acids transform between each other during life processes and play the important roles in the biological system. The research of the kinds and change of content of fatty acids can provide reference for some important disease. In recent years, chromatography has be widely used based on its fast development and excellent separation efficency. This paper reviews the analytical methods for determining fatty acids in home and abroad.