UPLC同时测定百灵安神片中七种活性成分含量

Simultaneous Determination of Seven Active Compounds in Bailing Anshen Tablets by UPLC

摘要: 目的建立UPLC同时测定百灵安神片中绿原酸、芒果苷、二苯乙烯苷、斯皮诺素、木犀草苷、槲皮苷、3，5-二咖啡酰奎宁酸含量的方法。方法采用Waters Acquity UPLC BEH C18(2.1 mm×100 mm，1.7 μm)色谱柱；流动相：乙腈-0.1%磷酸水，梯度洗脱；流速：0.4 mL·min^-1；检测波长：340 nm。结果绿原酸、芒果苷、二苯乙烯苷、斯皮诺素、木犀草苷、槲皮苷、3，5-二咖啡酰奎宁酸在检测范围内线性良好，r均>0.999 8，方法回收率分别96.3%，98.2%，100.5%，99.3%，97.7%，97.0%，96.4%。结论本方法准确可靠快捷，为全面控制百灵安神片质量提供了一种测定方法。

Abstract: OBJECTIVE To established a UPLC method for determination of seven active compounds in Bailing Anshen tablets, including chlorogenic acid, mangiferin, tetrahydroxystilbene, spinosin, cynaroside, quercitrin, and 3,5-dicaffeoylquinic. METHODS The chromatographic separation performed on a C18 column and with gradient elution of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.4 mL·min^-1. The detected wavelength was set at 340 nm. RESULTS The seven analytes showed good linearity (r>0.999 8) within the test ranges. The average recoveries were 96.3%, 98.2%, 100.5%, 99.3 %, 97.7%, 97.0%, 96.4%, respectively. CONCLUSION The analytical method is simple, rapid, specific, which is useful for quality control of Bailing Anshen tablets.