Abstract: OBJECTIVE To investigate the therapeutic effect of ganoderma triterpene(GT) on non-alcoholic fatty liver in mice. METHODS A model of the non-alcoholic fatty liver was constructed by feeding mice with high glucose and fatty diet for 5 weeks. Forty mice models with non-alcoholic fatty liver were randomly divided into five groups, model groups, Kezhi capsule groups(100 mg·kg^-1), GT groups (200, 100, 50 mg·kg^-1). The therapeutic effects of GT on non-alcoholic fatty liver and histopathological changes of mice liver were observed. RESULTS Compared with the non-alcoholic fatty liver model group, after treatment with GT, the mice serum levels of total cholesterol(TC), triglyceride(TG), alanine transaminase(ALT) and aspartate transaminase(AST) were significantly decreased(P<0.05 or P<0.01). The hepatic tissue contents of TC, TG and malondialdehyde(MDA) also significantly decreased (P<0.05 or P<0.01). The superoxide dismutase(SOD) activity was higher than that of mice in model group (P<0.05 or P<0.01). And the pathobiology of liver dramatically showed the repairing of hepatic steatosis. CONCLUSION GT has the hypolipideic function with liver protection on high fat diet-induced non-alcoholic fatty liver disease in mice.