十二碳烯酸对人H4细胞毒性作用研究

Cytotoxicity of 2-Dodecenoic Acid on Human H4 Cells

摘要: 目的探讨十二碳烯酸对人H4细胞的毒性作用机制。方法采用MTT法、流式细胞仪检测十二碳烯酸对人H4细胞的毒性作用。结果分别给予人H4细胞0.3，0.9，2.7 mg·L^-1十二碳烯酸培养48 h，十二碳烯酸对人H4细胞增殖抑制率分别为41.64%，70.97%，81.52%，半数抑制浓度(IC50)为0.40 mg·L^-1，与对照组相比，差异有统计学意义(P<0.01)；凋亡率分别为10.16%，18.22%，24.06%。结论十二碳烯酸对人H4细胞具有显著的毒性作用，其机制可能与损伤线粒体结构，抑制细胞分裂等有关。

Abstract: OBJECTIVE To study 2-dodecenoic acid toxicity mechanism on human H4 cells. METHODS Toxic effects of 2-dodecenoic acid on human H4 cells were detected with methods of MTT and flow cytometry. RESULTS Given the human H4 cells 0.3, 0.9, 2.7 mg·L^-1 2-dodecenoic acid for 48 h, human H4 cells proliferation inhibition rates were 41.64%, 70.97%, 81.52%, respectively, and the 50% inhibitory concentration(IC50) was 0.40 mg·L^-1, compared with the control group, the difference was statistically significant(P<0.01); apoptosis rates were 10.16%, 18.22%, 24.06%, respectively. CONCLUSION 2-Dodecenoic acid on human H4 cells has significant toxic effects, its mechanism may be related to the destruction of the cell mitochondrial structure and inhibition of cell division.