Abstract: OBJECTIVE To research Xihuang Pill drug chloroform extract of immune clearance function on Walker256 tumor-bearing rats, and to explore the mechanism of its anti-tumor effect. METHODS Seventy Wistar rats were randomly chosen 10 as control group, the other 60 rats established Walker256 model of tumor-bearing rats by subcutaneously injecting W256 tumor cells into rats and were randomly divided into negative control group, the control group of lentinan, Xihuang Pill durg high dose group, Xihuang Pill drug chloroform extract low, medium and high dose groups, in each experimental group administrated orally everyday. ELISA assay of interleukin 2, interferon-γ (IFN-γ) level of change; Changes of adhesion molecules B7-1, CD3⁺T, CD4⁺T, CD8⁺T cells in peripheral blood were observed by flow cytometry. The effects of Xihuang pill drug chloroform extract on immune clearance function in rats were also observed. RESULTS chloroform extract the midst dose group rat peripheral IL-2, IFN-γ, CD8⁺T cells, adhesion molecules B7-1 the percentage rise was significantly increased (P<0.05)compared with the negative control. Chloroform extracts the midst dose group and the low-dose group tumor-bearing rats in CD3⁺T cells in peripheral blood, CD4⁺T cells, was statistically significant difference(P<0.05) compared with the negative control group. CONCLUSION Xihuang Pill drug chloroform extract through the promotion of the proliferation and activation of T lymphocytes to enhance the body’s immune clearance of tumor-bearing function.