Abstract: OBJECTIVE To develop an HPLC method for determination of chlorogenic acid, forsythin, rhein, emodin and chrysophanol in Lianhuan Qingwen capsules. METHODS The sample was separated on an Agilent C₁₈ column(4.6 mm× 250 mm, 5 μm) with a mobile phase consisting of acetonitrile-0.1% acetic acid in gradient elution(0 min→10min→20min→30min→45min→53min, acetonitrile 10%→10%→20%→30%→68%→75%) at the flow rate of 1.0 mL?min^-1；at 0-15 min, the detection wavelength was 327 nm, at 15-35 min, the detection wavelength was 205 nm, at 35-43 min, the detection wavelength was 230 nm, at 43-48 min, the detection wavelength was 221 nm, at 48-55 min, the detection wavelength was 224 nm, and the column temperature was 32 ℃. RESULTS The results showed that all the components were well separated in 55 min by HPLC. Each main component chromatographic peak separation resolution was >2.0, the linear ranges and correlation coefficients were 0.444 5-7.112 μg(r=0.999 6), 0.167 8-2.684 μg(r=0.999 9), 0.031 0-0.496 3 μg(r=0.999 5), 0.031 5-0.504 μg(r=0.999 7), 0.065 5-1.048 μg(r=0.999 9), respectively. The sums of average recovery(n=9) were 102.2%(RSD=0.5%), 101.0% (RSD=0.8%), 98.32%(RSD=0.5%), 95.83%(RSD=0.6%), 102.65%(RSD=0.3%). CONCLUSION The method is convenient, accurate, and can be used for quantitative determination of the preparation.