HPLC-ELSD测定地黄寡糖中地黄苷A含量

Determination of Rehmannioside A in Rehmannia Glutinosa Oligosaccharide by HPLC-ELSD

摘要: 目的建立测定地黄寡糖中地黄苷A的高效液相色谱法。方法采用Agilent TC C₁₈色谱柱(4.6 mm×250 mm，5 μm)，柱温35 ℃，流动相：甲醇-水(10∶90)，流速1.0 mL·min^-1；ELSD参数：漂移管温度110 ℃,载气流量3.2 L·min^-1。结果地黄苷A在0.2~1.2 mg·mL^-1(r=0.999 3)内线性关系良好，平均回收率为98.8%(RSD=1.6%)。结论该方法准确、简便、专属性好，可用于地黄寡糖中地黄苷A成分的含量测定，为地黄寡糖的质量控制提供了依据。

Abstract: OBJECTIVE To establish an HPLC method to determine the content of rehmannioside A in Rehmannia glutinosa oligosaccharide. METHODS The HPLC-ELSD was used with Agilent TC C₁₈ column (4.6 mm×250 mm, 5 μm) at 35 ℃. The mobile phase was methanol and water(10∶90) with a flow rate 1.0 mL·min^-1; The temperature of the drift tube in ELSD was 110 ℃ and the flow rate of dry air was 3.2 L·min^-1. RESULTS The rehmannioside A had a good linear relationship between 0.2-1.2 mg·mL^-1. CONCLUSION The method is accurate, simple with good specificity，and it could be used to determine the content of rehmannioside A in Rehmannia glutinosa oligosaccharide, which may be providing a evidence for the quality control of Rehmannia glutinosa oligosaccharide.