Abstract: OBJECTIVE To investigate the skin permeability of diammonium glycyrrhizinate from differents vesicular carriers, and prepare vesicles gel. METHODS The phospholipid complex, enthosome and elastic liposome of diammonium glycyrrhizinate were prepared by aprotic solvent method, film dispersing and ethanol injection method, respectively. Particle sizes of the vesicles were determined. Skin permeation experiments were carried out on modified Franz diffusion cells, using excised human skin. The concentrations of diammonium glycyrrhizinate in the receptor compartment and skin were determined by HPLC. Finally, vesicle with good skin permeability was prepared as a gel agent, and its skin permeation was also determined. RESULTS The quantity of diammonium glycyrrhizinate from phospholipid complex in the receptor was (8.07±5.42)μg·cm^-2,while others were negligible. The cumulative amounts of drug in the skin after 24 h were in the order of that: phospholipid complex>enthosome>elastic liposome>water solution. Skin permeability of diammonium glycyrrhizinate phospholipid complex gel was affected by the concentrations of carbomer. The accumulation of diammonium glycyrrhizinate from phospholipid complex gel with 0.5% carbomer was 2.2 times higher than gel with 1% carbomer. Reduce the concentration of carbomer not only can improve glycyrrhizic acid content in the epidermis, but also make the drug to further penetrate into the dermis. CONCLUTIONS Phospholipid complex can significantly increase percutaneous penetration and skin cummulation of diammonium glycyrrhizinate. Phospholipid complex gel with 0.5% carbomer has a good percutaneous penetration.