醌茜素荷移分光光度法测定盐酸伊托必利

Spectrophotometry Determination of Itopride Hydrochloride Based on Charge-transfer Reaction with Quinalizarin

摘要: 目的建立测定盐酸伊托必利含量的方法。方法盐酸伊托必利与醌茜素在碱性条件下可发生电荷转移反应，形成稳定的1∶1型荷移络合物，在最大吸收波长578 nm处测定络合物的吸光度。结果盐酸伊托必利药物质量浓度在20.0~140.0 mg·L^-1内服从比耳定律，相关系数为0.999 1，RSD为0.62%，表观摩尔吸光系数为2.03×10³ L·mol^-1·cm^-1，回收率为99.1%~102.3%。结论方法准确、简便、快捷，可用于测定片剂中盐酸伊托必利的含量。

Abstract: OBJECTIVE To establish a method for determination of itopride hydrochloride. METHODS Itopride hydrochloride reacted with quinalizarin to form stable charge transfer complex whose composition ratio is 1∶1 under alkaline conditions and could be determined at 578 nm. RESULTS Beer′s Law is obeyed in the range of 20.0-140.0 mg·L^-1 of itopride hydrochloride and the correlation coefficient is 0.999 1. And RSD is 0.62%. The apparent molar absorptivity of the complex is 2.03×10³ L·mol^-1·cm^-1. The recoveries are 99.1%~102.3%. CONCLUSION The method is simple, rapid and accurate, can be used to the determination of itopride hydrochloride in tablets.