Abstract: OBJECTIVE To observe the effects of Hirudo on blood metabolism and its related gene expression in blood stasis syndrome rabbits. METHODS The blood stasis syndrome rabbit’s models induced by complex factors(starvation+ high-fat feeding+adrenaline) were established. Model rabbits randomly divided into five groups, rabbits in Hirudo groups were daily administered with Hirudo(0.30, 0.15, 0.075 g·kg^-1) for 30 days, and rabbits in the normal and model groups were daily administered with 0.9% NaCl. The serum TC, TG, LDL-C in each different treated rabbit group were determined after models established, 10, 20, 30 d administration, respectively. The mRNA expressions of LDL-R and ApoE in liver tissue were quantified by Realtime-PCR. RESULTS Compared with normol group, Hirudo groups could remarkably decrease the levels of serum TC, TG, LDL-C(P<0.05). The result of realtime-PCR demonstrated that the Hirudo could remarkly increase the mRNA expressions of LDL-R and ApoE in liver tissue of rabbit’s model group. CONCLUSION Hirudo has the effect of regulating lipld metabolism of blood stasis syndrome rabbits, the mechanism is likely related to increasing LDL-R and ApoE mRNA expression.