Abstract: OBJECTIVE To evaluate the effects of Xiaoer Jianpi cream on the contractile activity in ileum of guinea-pig and stomach muscle of rat in vitro, and to evaluate the effects of Xiaoer Jianpi cream on the contractile activity in stomach muscle of rat in situ and on the gastric emptying and intestinal propulsion in mice. METHODS In vitro and in situ experiments, the biological functional system was used to observe the effects of Xiaoer Jianpi cream on the contraction of ileum and stomach muscle. The gastric emptying and intestinal propulsion was researched by weighting method and labeling method in vivo. RESULTS In vitro experiment, different dosages of Xiaoer Jianpi cream increased the amplitude of contraction in guinea-pig ileum and stomach muscle of rat. In situ experiment, the large dosage and medium dosage of Xiaoer Jianpi cream could increase significantly the average contractile amplitude in stomach muscle of rat. There was a synergy between the large dosage group and neostigmine. In animal experiment, different doses of Xiaoer Jianpi cream had a significantly promotion on gastric emptying and intestinal propulsion in gastrointestinal disorder mice induced by atropine. CONCLUSION Xiaoer Jianpi cream has the effects on promoting gastrointestinal motility.