Abstract: OBJECTIVE To observe the effects of Wufu decoction on the immune regulation and tumor inhibition rate of tumor-burdened mice. METHODS Mice were randomly divided into six groups: model group, cyclophosphamide group, low, middle and high dose of Wufu decoction groups (5.25, 10.5, 21.0 g·kg^-1, equivalent to 10, 20 and 40 times of human clinical dosage), normal control group. RESULTS High and middle dose of Wufu decoction groups could significantly increase T lymphocyte transformations capacity induced by ConA(P<0.05), improve NK cell activity(P<0.05), and raise serum IFN-γ, TNF-α level(P<0.05). The tumor inhibition rates of three Wufu decoction groups were 11%-16%, but had no obvious difference compared with model group(P>0.05). CONCLUSION Wufu decoction has no significant inhibition to S180 transplantation tumor, but can significantly improve the immunological function of tumor-burdened mice.