香青兰总黄酮对哮喘大鼠白细胞介素-6、13、17和肿瘤坏死因子-α的影响

Effects of Moldavica Total Flavone on Interleukin-6, 13, 17 and Tumor Necrosis Factor-α Levels in Asthma Rats

摘要: 目的观察香青兰总黄酮对哮喘大鼠肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、13、17等细胞因子水平的影响。方法采用卵白蛋白致敏和激发建立大鼠哮喘模型，给予香青兰总黄酮进行干预后，ELISA法检测大鼠BALF中TNF-α、IL-6、IL-13和IL-17的含量。结果香青兰总黄酮180和360 mg·kg^-1·d^-1可降低哮喘大鼠BALF中TNF-α、IL-6、IL-13和IL-17含量(P<0.05或P<0.01)。结论香青兰总黄酮对哮喘TNF-α、IL-6、IL-13和IL-17等炎症因子的释放具有一定抑制作用。

Abstract: OBJECTIVE To investigate the effects of moldavica total flavone on interleukin-6, 13, 17 and tumor necrosis factor-α levels in ovalbumin-induced asthmatic rats. METHODS Rats asthma model was established by ovalbumin challenge methods. After therapy by moldavica total flavone, TNF-α, IL-6, IL-13 and IL-17 in BALF were detected respectively by ELISA. RESULTS The levels of TNF-α, IL-6, IL-13 and IL-17 in BALF were markedly decreased(P<0.05 or P<0.01) after administration of 180 and 360 mg·kg^-1·d^-1 moldavica total flavone in ovalbumin-induced asthmatic rats. CONCLUSION Moldavica total flavone can inhibit the release of inflammatory cytokines of TNF-α, IL-6, IL-13 and IL-17 on asthma.