HPLC-超滤离心法测定连翘酯苷复方脂质体包封率

Determination of Entrapment Efficiency of Forsythiaside Compound Liposome by HPLC-Ulrafiltration

摘要: 目的建立一种快速、准确测定连翘酯苷复方脂质体包封率的方法。方法采用逆向蒸发法制备连翘酯苷复方脂质体，建立检测连翘酯苷复方含量的高效液相色谱条件，用超滤离心法处理复方脂质体，测定连翘酯苷复方脂质体的包封率。结果用连翘酯苷和绿原酸对照品建立标准曲线，在0.781 25~50 μg·mL^-1内线性关系良好，采用超滤离心法处理样品后连续3次测定包封率，平均包封率为92.38%和44.72%，其RSD分别为2.76%和2.35%。结论 HPLC-超滤离心法可准确、方便的测定连翘酯苷复方脂质体的包封率。

Abstract: OBJECTIVE To establish a method for determining entrapment efficiency of forsythiaside compound liposome. METHODS The liposome were prepared by reverse evaporation method, and treated with ultrafiltration. HPLC was used to detect the content of drug in the forsythiaside compound liposome. RESULTS The standard curve for forsythiaside and chlorogenic acid was linear in the concentration range of 0.78 125-50 μg·mL^-1. The mean value entrapment efficiency of forsythiaside and chlorgenic acid in the three samples were 92.38% and 44.72% with RSD of 2.76% and 2.35%. CONCLUSION The entrapment efficiency of forsythiaside compound liposome can be determinated simply and quickly by the HPLC-ultrafiltration.