Abstract: OBJECTIVE To explore the influence propofol total intravenous anesthesia perioperative serum cytokines IL-2 and IL-6 in patients with biliary tract surgery. METHODS Thirty patients undergoing biliary surgery surgery were randomly divided into sevoflurane anesthesia (Group S ) and propofol total intravenous anesthesia group (Group P), 15 cases in each group. Serum samples were collected before induction of anesthesia (T0), surgery completed (T1), postoperative 24h (T2), postoperative 72h (T3), respectively. Serum levels of IL-2, IL-6 level were measured by ELISA method. RESULTS In T1 period, serum IL-2 of the two groups was significantly decreased compared with T0 (P<0.05), while in T1 and T2 period, serum IL-6 of the two groups was significantly higher (P<0.05). Compared with Group P, serum IL-6 of the Group S was significantly increased in T1(P<0.05). CONCLUSION The influence of sevoflurane and propofol on serum IL-2 level have no significant difference. Propofol total intravenous anesthesia significantly suppressed serum IL-6 levels, and inhibited the immune response induced by surgical stress.