Abstract: OBJECTIVE To establish an efficient microemulsion liquid chromatography(MELC) method and validate for simultaneous determination of five flavonoids (5,4′-dihydroxy-3,7,3′-trimethoxyflavone, rhamnatin, kumatakenin, ermanine and velutin) in Callicarpa kwangtungensis Chun.. METHODS The MELC separation was performed on a Hypersil BDS C_l8 column(4.6 mm×150 mm, 5 μm) with the mixture of 2.2% SDS-1.5% octane-7.75% n-butanol-0.5% triethylamine-88% water, the pH was adjusted to 3.7 with phosphoric acid as the mobile phase. The flow rate was set at 0.8 mL·min^-1 and the eluent was detected at 350 nm for the five flavonoids．RESULTS The calibration curves of the five flavonoids were linear (r≥0.999 5) over the concentration range of 1.520-72.25 μg·mL^-1. The mean recoveries were 99.6%-100.5%. CONCLUSION The results indicate that the optimized method is successfully applied to the analysis of five important flavonoids in Callicarpa kwangtungensis Chun..