Abstract: OBJECTIVE To investigate the stability of endostatin in PVC infusion bags, non-PVC infusion bags and drug infusion pumps. METHODS In a simulated clinical situation, endostatin was undergone appropriate dilution with 0.9% sodium chloride injection in PVC infusion bags, non-PVC infusion bags or drug infusion pumps. Then high-performance liquid chromatography (HPLC) was employed to determine endostatin concentrations at 0, 1, 2, 4 h or 0, 4, 8, 16, 24 h under 25 ℃ or 37 ℃. RESULTS Endostatin could keep stable in PVC infusion bags or non-PVC infusion bags for at least 4 h, and remained stable for 48 h in drug infusion pumps under 25 ℃ or 37 ℃. CONCLUSION In clinical practice, it is appropriate to administer endostatin by intravenous dripping or via automatic infusion pumps from the perspective of drug stability.