Abstract: OBJECTIVE To optimize the preparing condition of dihydroartemisinin-phospholipid complex. METHODS The single factor experiment and orthogonal design was used to optimize preparation technology of the complex by using the combining ratio as the assessment index. In addition, the content of dihydroartemisinin in dihydroartemisinin-phospholipid complex was determined by HPLC. RESULTS The optimal preparing conditions for dihydroartemisinin-phospholipid complex was the ratio of dihydroartemisinin to phospholipid of 1∶3, dihydroartemisinin concentration of 5%, the reaction time of 3 h, and the solvent of chloroform and the reaction temperature at 55 ℃. CONCLUSION Dihydroartemisinin-phospholipid complex was affected by solvent, the ratio of dihydroartemisinin to phospholipid, dihydroartemisinin concentration and reaction time at 55 ℃.