Abstract: OBJECTIVE To establish the method for determining the content of taxfolin and quercetin in fructus polygoni for its quality standards to provide the scientific basis for the study. METHODS The Agilent TC-C₁₈ Column(4.6 mm×250 mm, 5 μm) was used, phase A was 0.1% phosphoric acid and phase B was methanol∶acetonitrile (60∶40) as the mobile phase, gradient elution, where phase B was mixed by methanol and acetonitrile in 60∶40 volume ratio of mixed organic phase. The flow rate was 1.0 mL·min^-1, the detection wavelength 290 nm in 0-42 min, 371 nm in 42-49 min, the column temperature was 25 ℃. RESULTS The isolation effect among taxfolin and quercetin showed good linear correlation, the average recoveries were 99.89%, 99.22%; RSD were 2.03%, 3.00%. CONCLUSION There have a great differences in contents of taxifolin and quercitrin among the samples from different origins. The method is simple, accurate and with good reproducibility. Which could be used as fructus polygoni orientalis quality control of medicines, it also provide a scientific reference for the rational use of fructus polygoni orientalis.