Abstract: OBJECTIVE In order to make clinical use of antibacterial more rational, intervention of antimicrobial agents duration cesarean perioperative was applied. METHODS 360 cases of selective cesarean section were randomly selected from our hospital during Jun.-Dec. in 2009 and Jun.-Dec. in 2010 and Jun.-Dec. in 2011, respectively(n=120), before, during and after intervention. Retrospective analysis was applied to analyze the application of antimicrobials in those cases. RESULTS The drug of choice, Dosage, initial administration time, duration, etc after the intervention has been significantly improved. CONCLUSION The implementation of the intervention can significantly increase the cesarean section perioperative antibiotic use the proportion of promoting the hospital safe, effective, economical and rational use of antimicrobial agents play a positive role in promoting.