Abstract:
OBJECTIVE To determine the content of Patchouli alcohol in Huoxiang Zhengqi Dripping Pill by GC.
METHODS The GC separation was performed on capillary gas chromatographic column; FID flame ionization detector; carrier gas is nitrogen; temperature setting: sampling input temperature 280 ℃; column temperature 165 ℃,temperature programming process; detector temperature 280 ℃; split ratio: 5∶1; sample size: 1 μL.
RESULTS In the GC experiments of precision, reproducibility and stability, the samples could be separated entirely. For patchouli alcohol, the linear range was 0.030 0-0.961 0 μg(
r=0.999 9), the average recoveries were 101.1% with RSD=1.45% and it was stable in 24 h.
CONCLUSION The method was sensitive, simple, accurate and reproducible, can be used to control the quality of Huoxiang Zhengqi Dripping Pill.