Abstract: OBJECTIVE To establish a method for determination of trace elements in Fritillariae Thunbergii Bulbus by ICP-OES, and compare 18 trace elements content of Fritillaria from different places, such as Ag, As, B, Ba, Bi, Cd, Co, Cr, Cu, Fe, Ga, Hg, In, Mn, Ni, Pb, Tl, Zn. METHODS Microwave digestion procedure was applied under optimized conditions for digesting Fritillaria. Concentrations of elements were determined by ICP-OES. RESULTS Experimental results showed that linear relationship was good, r was between 0.999 3 and 0.999 9. Average recovery rate was between 95.0% and 103.2%, RSD was less than 10%(n=6). The results met requirement of microanalysis. CONCLUSION Microwave digestion is a simple and rapid method. It digests completely with low blank value and the measurement result is satisfactory. ICP-OES method is rapid, accurate and convenient, can be used to determine various trace elements.