HPLC测定化妆品中非法添加的米诺地尔

Determination of Minoxidil Illegally Adulterated in Cosmetics by HPLC

摘要: 目的建立快速鉴定化妆品中非法添加的米诺地尔的检测方法。方法以迪马C18柱(4.6 mm×250 mm，5 μm)为分析柱；采用甲醇-水-冰醋酸(730∶270∶10)(1 000 mL^-1中含磺基丁二酸钠二辛酯3.0 g，并用高氯酸调节pH值至3.0)为流动相；流速为1.0 mL·min^-1；检测波长为280 nm。结果米诺地尔在1~100 μg·mL^-1浓度内线性良好；最低检测限为5 μg·g^-1；平均回收率100.4%。结论本法快速准确、专属性强、灵敏度高，可用于育发类化妆品中米诺地尔的检测。

Abstract: OBJECTIVE To establish a specific method for the determination of minoxidil illegally adulterated in cosmetics. METHODS The separation and analysis was performed on a Diamonsil C18 column(4.6 mm×250 mm, 5.0 μm). The mobile phase consisted of methanol-water-acetic acid glacial (730∶270∶10)(contained 0.3% of dioctyl sodium sulfosuccinate and adjusted pH to 3.0 with perchloric acid). The flow rate was 1.0 mL·min^-1 and detected at 280 nm. RESULTS The assay linearity of minoxidil was confirmed over the range of 1-100 μg·mL^-1, and the detection limit was 5 μg·g^-1. The average recovery was 100.4%. CONCLUSION The method is proved to be accurate, specific, sensitive and convenient. Therefore, it can be used to determinate minoxidil illegally adulterated in hair cosmetics.