总黄酮、高良姜素和白杨素含量指标综合优选蜂胶前处理方法

Comprehensive Evaluation on Pretreatment Methods of Propolis by Determination of Total Flavonoids, Galangin and Chrysin Content

摘要: 目的建立蜂胶含量分析的最佳前处理方法。方法采用单因素及L₉(3⁴)正交设计试验，以总黄酮、高良姜素、白杨素成分的含量变化为评价指标，对各流程进行优化，筛选最佳前处理方法。结果最佳前处理方法：药材粉末用95%乙醇50 ℃超声提取2次，每次30 min。结论该方法准确可靠，能作为蜂胶含量分析前处理方法。

Abstract: OBJECTIVE To establish optimal propolis pretreatment methods by comprehensive evaluation. METHODS Single-factor and orthogonal experiment was designed with the content change of total flavonoids, galangin and chrysin as evaluation index to screen the best pretreatment methods. RESULTS The optimal pretreatment condition was as follows: 95% ethanol was used as extraction solvent, ultrasonic extraction was applied at 50 ℃ for 2 times, 30 minutes for each time. CONCLUSION The method was scientific and reasonable which can be used in pretreatment of propolis.