高级检索

    HPLC测定参芪生肌颗粒中隐丹参酮和丹参酮ⅡA的含量

    Determination of Cryptotanshinone and Tanshinone ⅡA in Shenqi Shengji Granule by HPLC

      摘要
    • 摘要: 目的 建立HPLC测定参芪生肌颗粒中隐丹参酮和丹参酮ⅡA含量的方法。探讨参芪生肌颗粒的质量控制标准。 方法 采用HPLC测定，C18柱分离；柱温：35 ℃；流动相：甲醇-水(75∶25)；流速：1.0 mL·min-1；检测波长：270 nm。 结果 隐丹参酮和丹参酮IIA线性范围分别为8.016~48.096 μg(r=0.999 9)和16.025 6~96.153 6 μg(r=0.999 9)。平均回收率分别为98.7%和98.6%，RSD分别为0.9%和1.5%(n=9)。 结论 此方法简便、快速准确、灵敏度高、重现性好。可以更有效地控制参芪生肌颗粒制剂的质量。

       

      Abstract: OBJECTIVE To establish an HPLC method for determination of cryptotanshinone and tanshinone ⅡA in Shenqi Shengji granule, and to discuss the quality control standard of Shenqi Shengji granule. METHODS The samples were separated and determined by HPLC method. The column of C18 with temperature at 35 ℃ was used to separate the target components. The eluate composed of methyl alcohol-water (75∶25), the flow rate was 1.0 mL·min-1 and the detection wavelength was 270 nm. RESULTS The good linear relationships were obtained in the following range, cryptotanshinone 8.016-48.096 μg(r=0.999 9) and tanshinone ⅡA 16.025 6-96.153 6 μg(r=0.999 9). The average recoveries were 98.7% and 98.6%, respectively, and RSDs were 0.9% and 1.5%(n=9). CONCLUSION This method is simple, sensitive and reliable. The method provides a better way to control the quality of the Shenqi Shengji granule.

       

      • HTML全文
      • 参考文献(1)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回