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    HPLC测定怀地黄中桃叶珊瑚苷的含量

    Content Determination of Aucubin in Huai-Rehmannia Radix by HPLC

      摘要
    • 摘要: 目的 测定不同品种、不同产地怀地黄中桃叶珊瑚苷的含量，初步对怀地黄种质资源进行评价。方法 采用HPLC，Dikma Diamonsil C18色谱柱(4.6 mm×250 mm，0.5 μm)，流动相为甲醇-水(5.5∶94.5)，检测波长为206 nm。结果 桃叶珊瑚苷在0.053 5~1.284 μg间线性关系良好，回归方程为Y=539 696X+3 581.6(r=0.999 9)，回收率分别为99.75%(鲜地黄)和99.61%(生地黄)，RSD分别为2.86%和2.15%(n=6)；不同品种、不同产地怀地黄中桃叶珊瑚苷含量不同。结论 本法操作简单、灵敏、稳定，适用于怀地黄中桃叶珊瑚苷的含量测定。

       

      Abstract: OBJECTIVE To analyse the aucubin content in Huai-Rehmannia Radix from different varieties and habitats and to preliminarily evaluate the germplasm resources. METHODS HPLC method was used with the column packed with Dikma Diamonsil C18(4.6 mm×250 mm, 0.5 μm), the mobile phase was a mixture of methanol and water in a ratio of 5.5∶94.5, the detection wavelength was 206 nm. RESULTS The calibration curve of aucubin was in good linearity over the range of 0.053 5-1.284 μg and the regression equation was Y=539 696X+3 581.6(r=0.999 9), the average recovery was 99.75%(n=6, RSD 2.86%, fresh Rehmannia Radix) and 99.61%(n=6, RSD 2.15%, dried Rehmannia Radix) respectively; the contents of aucubin in Huai-Rehmannia Radix from different varieties and habitats were different. CONCLUSION The method has the advantage of simple operation, sensibility and stability. It can be applied to the content determination of aucubin in Huai-Rehmannia Radix.

       

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